Genomic Surveillance and Pandemic Preparedness offers practical, in-depth instruction in where, how, and why genomic surveillance may be applied. Drawing heavily from the learnings during the COVID-19 pandemic, this book covers different aspects of microbes with a focus on viral genome sequencing and analysis, implementation and inference of genomic surveillance, and global data sharing best practices for future pandemic preparedness. Here, more than a dozen international authors consider host and pathogen genome interaction, population-level prevalence, individual disease susceptibility and gene protection, pathogen mutation evolutionary dynamics, RNA modulation of infection, pathogen diversity, and the role of coinfections and comorbidities in disease severity. A wide range of examples, from bacterial pathogens to fungal and viral infections, with an emphasis on recent COVID-19 analysis, are discussed. Finally, the authors provide step-by-step instruction in experimental and computational approaches for genomic surveillance, including nucleic acid isolation, sequencing library preparation, functional sequencing analysis, and bioinformatics pipelines. Throughout this book, importance is placed on inference—moving from genomic sequencing to meaningful genomic surveillance that bolsters pandemic preparedness.
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